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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-20-176160 |
MA1-075 |
Hamster Anti-LAP 2 Monoclonal Antibody, Unconjugated, Clone RL29/200 ul/Rat/Hamster |
1/EA |
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Subject
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Hamster Anti-LAP 2 Monoclonal Antibody, Unconjugated, Clone RL29 |
Description
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200 ul / Rat/Hamster |
Clonality
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Mono |
Company
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Thermo Scientific |
Application
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Immunofluorescence, Immunoprecipitation, Western Blot |
Conjugation
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Unconjugated |
Immunogen
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LAP 2, Lamin Associated Polypeptides |
Contents
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Immunogen: Pore complex-lamina fraction isolated from rat liver nuclear envelopes. MA1-075 detects lamin associated polypeptide (LAP) 2 from various rat tissues but does not cross-react with LAP 1A, 1B & 1C. MA1-075 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody recognizes a single 53 kDa protein representing LAP 2 in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 2 in rat liver with MA1-075 results in labeling of the nuclear periphery and chromosome surfaces. MA1-075 antigen is pore complex-lamina fraction isolated from rat liver nuclear envelopes.In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins. The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B & 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A & B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly. |
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