AffiniClear secondary antibodies are conjugated to HRP and optimized for ECL applications. These antibodies react with the heavy and light chains of the immunoglobulin molecule. Pre-absorbed with the following species proteins: Bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins.AffiniClear secondary antibodies are conjugated to Horseradish-Peroxidase (HRP) for use in Western blot applications. These antibodies are affinity-purified and optimized for use in ECL experiments and have been absorbed against the serum proteins of a large number of species to virtually eliminate background and non-specific staining.