| Immunogen | Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line. |
| Product Type | Antibodies |
| Recommended Isotype Control | AM03095PU-N |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Magor, K.E. et al. (1994) One gene encodes the heavy chains for three different forms of IgY in the duck. J. Immunol. 153: 5549-5555. 2. Higgins, D.A. and Warr, G.W. (1993) Duck immunoglobulins: structure, functions and molecular genetics. Avian Pathol. 22: 211-236. |
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| Format | State: Liquid purified IgG |
| ? | Purification: Affinity chromatography on Protein G |
| ? | Buffer System: PBS containing 0.09% Sodium Azide |
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| Specificity | This antibody recognises both the IgY and IgY(-Fc) heavy chain molecules. This antibody also detects IgY heavy chain in Mallard ducks, goose and swan. This antibody detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under nonreducing conditions in Western blotting. |
| Application | Flow Cytometry. ELISA. Western Blot under non-reducing conditions. |
| Background | The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab)2 fragment of IgY. |
| Concentration | 0.5 mg/ml |
