| Immunogen | Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line. |
| Product Type | Antibodies |
| Recommended Isotype Control | AM03095PU-N |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev. and Comp. Immunol. 29: 733-748. 2. Schneider, K. et al. (2004) Chicken BAFF - a highly conserved cytokine that mediates B cell survival. Int. Immunol. 16: 139-148. |
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| Format | State: Liquid purified IgG |
| ? | Purification: Affinity chromatography on Protein G |
| ? | Buffer System: PBS containing 0.09% Sodium Azide |
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| Specificity | This antibody recognises the light chain of duck immunoglobulin. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting. |
| Application | Flow Cytometry. ELISA. Western Blot. |
| Background | IgY Light Chain is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. |
| Concentration | 1.0 mg/ml |
