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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-310811 |
NB600-540 |
Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49)//Bovine(-), Can/Mouse |
1/EA |
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Subject
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Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) |
Description
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/ Bovine(-), Can/Mouse |
Clonality
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Mono |
Company
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Novus biologicals |
Application
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FACS, IP |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Sh(-), Gt(-), Fe, Gp(-), Rb, Rt(-), Po, Mu(-), Hu, Ha(-), Ca(-), Bv(-) | Applications: | IP, FACS | Clonality: | Monoclonal | | Gene: | ALPL | Purity: | Affinity purified | Host: | Mouse | | Specificity: | This does not cross react with other isozymes of AP. | Publications: | Antibody has not yet been mentioned in a publication. | Details: Clone: | TRA-2-49 | Isotype: | IgG1 | Immunogen: | 2102Ep Human Embryonal Carcinoma Cells | Epitope: | Liver/Bone/Kidney Alkaline Phosphatase | Localization: | Cell Membrane | Gene Symbol: ALPL Entrez: | 249 (Human) | | 11647 (Mouse) | | 25586 (Rat) | Related Genes: | ALPP, SLPI, CCL27, PDLIM3, ASRGL1, ATRNL1, NAT10, SPP1, ENPP1, ALPI, SNARE, ANKH, RUNX2, GPI, ACHE, GNPDA1, PLP1, PRDX5, PDXP, PTHLH, PPA1, IAPP, MAGT1, CD47, HOPX, STIP1, ANK1, PLAA, LNPEP, ST13, BGLAP, PHEX, VPS39, DMD, CXXC1, ALB, BEST1, TWIST1, IL1RL1, SULT2A1 | Background: Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry. Species: Reactivity: | Human, higher primates, rabbit, cat, pig and tiger. Does not react with mouse, rat, old world monkeys (including Rhesus Monkeys and Marmosets), bovine, canine, goat, hamster, sheep or guinea pig proteins. Other species have not been tested. | Applications: Uses: | This antibody is useful for FACS/Flow cytometry and immunoprecipitation. | Dilutions: | flow cytometry / FACS analysis - 5-20 ug/ml, immunoprecipitation , | Concentrations: | 1.0 mg/ml | Positive Controls: | 1 positive controls | Packaging: Buffer: | 0.02M PBS, 0.25M sodium chloride, [pH7.6] | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | Preservative: | 0.1% Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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