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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-311060 |
NB600-536 |
Actin Alpha 2 Smooth Muscle Antibody (1A4)//Bb, Human,/Mouse |
1/EA |
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Subject
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Actin Alpha 2 Smooth Muscle Antibody (1A4) |
Description
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/ Bb, Human,/Mouse |
Clonality
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Mono |
Company
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Novus biologicals |
Application
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ELISA, ICC, IF, IHC-P, Western Blot |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Bb, Rb, Rt, Mk, Hu | Applications: | ICC, WB, IF, IHC, ELISA | Clonality: | Monoclonal | | Gene: | ACTA2 | Purity: | Tissue culture supernatant | Host: | Mouse | | Specificity: | This reacts with the alpha smooth muscle isoform of actin. It does not react with actin from fribroblasts, striated muscle and myocardium. This reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary. | Publications: | Antibody has not yet been mentioned in a publication. | Details: Clone: | 1A4 | Isotype: | IgG2a Kappa | Immunogen: | The N-terminal decapeptide of alpha-smooth muscle actin. | Localization: | Cytoplasmic | Gene Symbol: ACTA2 Entrez: | 59 (Human) | | 287876 (Rat) | Related Genes: | MYH7, DES, ACTC1, NPPA, MYOD1, MYOG, MYH14, MYL1, NKX2-5, ACTA1, PLN, TTN, TGFBR1, GJA1, CDKN1A, TNNT2, HNRNPC, C2, PCNA, LMNA, MLC1, TGFBR2, HESX1, ELSPBP1, SGCD, HAND1, MSTN, GATA4, VCL, PSMD1, MYC, MYF5, MYF6, NOL3, VCAN, MYH11, FBN1, TGFB1, MSC, TNFRSF11A | Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Species: Reactivity: | Cross-reacts with Human, Rabbit, Rat, Pig, and Baboon. Not yet tested in other species. | Applications: Uses: | This antibody is useful for IHC, Western Blot, immunofluorescence and ELISA. Immunohistochemistry (formalin fixed paraffin embedded or frozen): 1/50 - 1/75 (30 minutes at RT). Immunocytochemistry: 1/75 Immunofluorescence: 1/25 -1/50 (cells) and 1/100 (vessel sections) This antibody can also be used on frozen tissue sections for IHC. | Dilutions: | ELISA 1/200 - 1/1, immunohistochemistry , immunofluorescence , Western Blot 1/100 - 1/300, Immunocytochemistry | Concentrations: | 0.2 mg/ml | Packaging: Buffer: | 20 mM tris-borate, 150 mM sodium chloride, | Storage: | Store at 4C. Do not freeze. | Preservative: | Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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