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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-311080 |
NB300-721 |
Alpha Adaptin A/C Antibody (AC1-M11)//Am, Bovine,/Mouse |
1/EA |
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문의 |
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Subject
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Alpha Adaptin A/C Antibody (AC1-M11) |
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Description
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/ Am, Bovine,/Mouse |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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IF, IP, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Am, Mk, Ch, Po, Bv, Mu, Rt, Hu | | Applications: | WB, IP, IF | | Clonality: | Monoclonal | | | Gene: | AP2A1 | | Purity: | Ascites | | Host: | Mouse | | | Specificity: | Detects alpha Adaptin. This detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons. | | Publications: | Antibody has been mentioned in at least 1 publication. | Details: | Clone: | AC1-M11 | | Isotype: | IgG2a | | Immunogen: | Purified bovine brain adaptor complexes. | | Localization: | Cytoplasmic | Gene Symbol: AP2A1 | Entrez: | 160 (Human) | | | 11771 (Mouse) | | | 308578 (Rat) | | Related Genes: | TFAP2A, PIGR, SLC2A4, FABP4, USF1, CFD, GTF3A, NOTCH, C/EBP, GSTK1, CARD16, SLCO6A1, SH3D19, INS, GAPDH, USF2, NUMB, SP3, SP1, GLB1, PPARG, SHC1, PSG1, COPE, EBP, COPB2, DAND5, COPB1 | Background: Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression. Species: | Reactivity: | Cross-reacts with Human, Rat, Mouse, Cow, Pig, Chicken, Primate and Amphibians. Not yet tested in other species. | Applications: | Uses: | Immunofluorescence, Western Blot, Immunoprecipitation Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells yields distinct punctate dots on the plasma membrane. | | Dilutions: | immunofluorescence 1:100, immunoprecipitation Assay dependent, Western Blot 1:100 | Packaging: | Buffer: | PBS | | Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. | | Preservative: | 0.05% Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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