랩캠퍼스(Labcampus) : [항체] - active/cleaved Caspase 7 Antibody

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active/cleaved Caspase 7 Antibody

Brand : Novus biologicals

Cat. No	Model	Description	Unit	Price (VAT별도)	납기	재고	DataSheet	주문/견적
LCA-23-312194	NB100-56115	active/cleaved Caspase 7 Antibody//Human, Mouse,/Rabbit	1/EA					문의

Subject

active/cleaved Caspase 7 Antibody

Description

/ Human, Mouse,/Rabbit

Clonality

Poly

Company

Novus biologicals

Application

IHC-P, IP, Western Blot

Conjugation

Immunogen

Contents

Description:Summary:

Species:	Rt, Mu, Hu
Applications:	IHC-P, WB, IP
Clonality:	Polyclonal

Gene:	CASP7
Purity:	Whole antisera
Host:	Rabbit

Specificity:

This polyclonal antisera recognizes the large (~17-22 kDa) and small (~10 kDa) subunits of active/cleaved caspase-7. Whereas the antisera has a strong preference for active/cleaved caspase-7, in some cell or tissue systems or techniques the antisera may also recognize the proform of Caspase-7 (~35 kDa).

Details:

Isotype:	IgG
Immunogen:	Recombinant catalytically active human Caspase-7 protein was used as immunogen.

Gene Symbol: CASP7

Entrez:	840 (Human)
	64026 (Rat)
Related Genes:	CASP3, CASP9, CASP8, PARP1, CASP6, CYCS, BCL2, COL11A2, XIAP, BID, TP53, FAS, CASP2, BCL2L1, BAX, CASP1, TNFSF10, MAPK1, CDKN1A, APAF1, TNF, AKT1, FASLG, GZMB, MAPK8, ANXA5, DIABLO, MAPK3, ALPI, CD47, IAPP, MAGT1, CASP10, TCEAL1, D4S234E, HSPA5, MAPK14, BAK1, AHSA1, PIK3CG

Background: Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-7 polyclonal antisera recognizes the large (approx. 17-22 kDa) and small (approx. 10 kDa) subunits of active/cleaved caspase-7. Whereas the antisera has a strong preference for active/cleaved caspase-7, in some cell or tissue systems or techniques the antisera may also recognize the proform of Caspase-7 (approx. 35 kDa). Species:

Reactivity:

Cross reacts with Gerbil, Human, Mouse and Rat.

Applications:

Uses:	Immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Dilutions:	immunoprecipitation 1:50-1:200, Western Blot 1:1000-1:2000, Immunohistochemistry-Paraffin 1:1000-1:5000
Positive Controls:	1 positive controls

Packaging:

Buffer:	PBS
Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative:	0.05% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.