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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-315634 |
NB100-1629 |
E2F1 Antibody//Human/Rabbit |
1/EA |
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¹®ÀÇ |
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Subject
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E2F1 Antibody |
Description
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/ Human/Rabbit |
Clonality
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Poly |
Company
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Novus biologicals |
Application
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ELISA, IP, Western Blot |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Hu | Applications: | ELISA, WB, IP | Clonality: | Polyclonal | | Gene: | E2F1 | Purity: | Immunogen affinity purified | Host: | Rabbit | | Specificity: | E2F1 | Publications: | Antibody has not yet been mentioned in a publication. | Details: Isotype: | IgG | Immunogen: | Synthetic peptide: KTPSQEVTSEEENRATDSAT conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 310-329 of Human E2F 1. | Localization: | Nuclear | Gene Symbol: E2F1 Entrez: | 1869 (Human) | Related Genes: | ARID4A, CDKN1A, CDKN2A, TP53, CDK2, E2F4, RB1, CDK4, E2F3, E2F2, CCND1, TCEAL1, D4S234E, RBL1, MYC, RBL2, TFDP1, CCNA2, PCNA, RAB3GAP1, NOLC1, E2F5, PAK3, DCTN6, SSSCA1, IFI27, PSMD9, MDM2, SLC12A9, CDKN1B, BCL2, DHFR, CDC2, CASP3, TP73, SP1, ARHGAP24, NOL3, BAX, DAND5 | Background: Anti-E2F1 reacts specifically with the E2F1 transcription regulator protein (recombinant). The antibody may be used for immunoblotting (~50 kDa) and immunoprecipitation techniques using whole cell extracts of E2F1 transfected cells, or in vitro translated E2F1 proteins. Staining of the E2F1 band by immunoblotting is specifically inhibited with the immunizing E2F1 peptide. The E2F family of transcription factors is important for cell cycle control. The E2F transcription factors are heterodimeric molecules composed of two distinct proteins termed E2F and DP. The E2F component of this protein complex is encoded by a family of genes consisting of at least 6 members (E2F-1 to -6). The DP family contains three proteins (DP -1 to -3), which heterodimerize interchangeably with any of the E2F proteins. E2F/DP target genes include genes important for DNA replication (e.g. DHFR, ORC1, MCM), cyclin genes (A and E), proto-oncogene (myc, myb) and the Rb family genes (Rb, p107). The E2F/DP transcription factors are tightly regulated by multiple regulatory mechanisms. Besides being regulated by the combination of the two components, the E2F/DP complex is negatively regulated by binding to one or more members of the retinoblastoma (Rb) pocket protein family: pRb, p107 and p130. Complexes of unphosphorylated pRb and E2F/DP act as transcriptional repressors that contribute to the Rbdependent G1 arrest. Once a pocket protein is phosphorylated by CDKs, the E2F/DP/pocket protein complex dissociates, resulting in free, transcriptionally active E2F/DP heterodimer. Another level of regulation is the synthesis and degradation of the proteins. Certain E2F species are actively degraded by the ubiquitin-proteasome pathway and their synthesis is cell-cycle regulated. The E2F proteins can be grouped into 3 subfamilies based upon structural homology and functional characteristics. The first subfamily consists of E2F-1, -2, and Species: Reactivity: | Cross-reacts with Human. Not yet tested in other species. | Applications: Uses: | Immunoprecipitation, Western Blot and Microarray: In Western BLot a 50kDa band is seen. Optimal dilutions should be determined by the end user. | Dilutions: | immunoprecipitation assay dependent, Western Blot 2.5 ug/mL , ELISA, | Packaging: Buffer: | 10mM PBS. [pH 7.4] with 1% BSA | Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. | Preservative: | 15mM Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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