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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-317757 |
NB100-63173 |
6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP]//ELISA, WB/Mouse |
1/EA |
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문의 |
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Subject
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6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] |
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Description
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/ ELISA, WB/Mouse |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Applications: | WB, ELISA | | Clonality: | Monoclonal | | | Purity: | protein G purified | | Host: | Mouse | | | Specificity: | This antibody recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. | | Publications: | Antibody has not yet been mentioned in a publication. | Details: | Conjugate: | HRP | | Clone: | AD1.1.10 | | Isotype: | IgG1 | | Immunogen: | PAX6 transcription factor linked to histidine tag | Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Species: | Reactivity: | Cross reacts with synthetic peptide. | Applications: | Uses: | This antibody is useful for ELISA and Western Blot. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. This product is routinely tested in ELISA and Western blotting on histidine tagged ubiquitin. | | Dilutions: | ELISA Western Blot | | Concentrations: | 1.0 mg/ml | | Conjugated Ab's: | 2 conjugated antibodies | Packaging: | Buffer: | Phosphate buffered saline, pH7.4 | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 0.01% Thimerosal | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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