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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-317759 |
NB100-64151 |
6-Histidine Epitope Tag Antibody (AD1.1.10) [PE]//FACS/Mouse |
1/EA |
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Subject
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6-Histidine Epitope Tag Antibody (AD1.1.10) [PE] |
Description
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/ FACS/Mouse |
Clonality
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Mono |
Company
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Novus biologicals |
Application
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Conjugation
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Immunogen
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Contents
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Description:Summary: Applications: | FACS | Clonality: | Monoclonal | | Purity: | protein G purified | Host: | Mouse | | Specificity: | Recognizes proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. | Publications: | Antibody has not yet been mentioned in a publication. | Details: Conjugate: | PE | Clone: | AD1.1.10 | Isotype: | IgG1 | Immunogen: | PAX6 transcription factor linked to histidine tag | Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Species: Reactivity: | Synthetic Peptide | Applications: Uses: | Flow cytometry / FACS analysis | Dilutions: | flow cytometry / FACS analysis 1:5 | Concentrations: | 0.1 mg/ml | Conjugated Ab's: | 2 conjugated antibodies | Notes: Reconstitute with 1.0ml of distilled water. Packaging: Buffer: | Phosphate buffered saline pH7.4 and 1.0% BSA lyophilised. | Storage: | Store at 4C. Do not freeze. | Preservative: | 0.09% Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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