| Antigen: human 11β-Hydroxysteroid dehydrogenase (Type 2) (11β-HSD2) amino acids 25-40 (RSDLRLGRPLLAALAL) · Host: rabbit · Application(s): WB and ICC; other applications not tested · 11β-HSD2 plays a critical role in normal physiology in the corticosteroid regulation of sodium homeostasis and the pathophysiology of hypertension by converting active cortisol to inactive cortisone. Mutation in the gene encoding 11β-HSD2 results in cortisol induction of hypertension and hypokalemia, a syndrome of apparent mineralocorticoid excess.1 11β-HSD2 mRNA and protein are expressed in placenta and mineralocorticoid target tissues, particularly the kidney.2 In mouse, expression of this enzyme is tissue-specific and developmentally regulated, indicating the importance of the enzyme in controlling glucocorticoid-mediated maturation.3 Cayman’s 11β-HSD2 polyclonal antibody can be used for western blotting and immunochemical analysis of 11β-HSD2 from samples of human, rat, and mouse. The antibody detects a band at 44 kDa in tissues such as kidney and cell lines including Caco-2 cells and HT-29 cells. |
| 1 Quinkler, M., Stewart, P.M. Hypertension and the cortisol-cortisone shuttle. Journal of Clinical Endocrinology & Metabolism 88(6) 2384-2392 (2003). 2 White, P.C., Mune, T., Agarwal, A.K. 11β-hydroxysteroid dehydrogenase and the syndrome of apparent mineralocorticoid excess. Endocr Rev 18(1) 135-156 (1997). 3 Thompson, A., Han, V.K.M., Yang, K. Differential expression of 11β-hydroxysteroid dehydrogenase types 1 and 2 mRNA and glucocorticoid receptor protein during mouse embryonic development. J Steroid Biochem Molec Biol 88 367-375 (2004). |
