| Antigen: Acetylated KLH · Clone designation: 7F8 · Host: Mouse · Isotype: IgG1 · Application(s): ELISA, WB, and ICC · This antibody is useful for monitoring levels of acetylation (i.e., histone and p53).1 Histone subunit modifications such as lysine acetylation are regulated by the activity of histone acetyltransferases (HATs) and histone deacetylases (HDACs).2,3 Epigenetic modifications directly influence cellular genetic programs including those contributing to cancer cell viability.4,2,5 This monoclonal antibody was generated using acetylated KLH as an immunogen. |
| 1 Luo, J., Nikolaev, A.Y., Imai, S., et al. Negative control of p53 by Sir2α promotes cell survival under stress. Cell 107 137-148 (2001). 2 Loidl, P. Histone acetylation: Facts and questions. Chromosoma 103 441-449 (1994). 3 Cheung, W.L., Briggs, D.B., Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1). 4 Marks, P.A., Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nature Biotechnology 25(1) 84-90 (2007). 5 Bolden, J.E., Peart, M.J., Johnstone, R.W. Anticancer activities of histone deacetylase inhibitors. Nature Reviews Drug Discovery 5 769-784 (2006). |
