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| Cat. No |
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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-311404 |
NB110-57447 |
B Raf Antibody (EP152Y)//Human, Mouse(-)/Rabbit |
1/EA |
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문의 |
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Subject
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B Raf Antibody (EP152Y) |
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Description
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/ Human, Mouse(-)/Rabbit |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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FACS, IHC-P, IP, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt, Mu(-), Hu | | Applications: | WB, IP, IHC, FACS | | Clonality: | Monoclonal | | | Gene: | BRAF | | Purity: | IgG purified | | Host: | Rabbit | | | Specificity: | A synthetic peptide corresponding to residues in human B-Raf was used as immunogen. | | Publications: | Antibody has not yet been mentioned in a publication. | Details: | Clone: | EP152Y | | Isotype: | IgG | Gene Symbol: BRAF | Entrez: | 673 (Human) | | | 109880 (Mouse) | | | 114486 (Rat) | | Swiss Prot: | P15056 (Human) | | Related Genes: | RAF1, MAPK1, ZHX2, EPHB2, KRAS, CDKN2A, HRAS, MAPK3, TP53, EGFR, NRAS, MAP2K1, MLH1, SNRPE, PIK3CG, PIK3CA, AKT1, RET, MAP2K2, PTEN, CTNNB1, MAPK12, ARAF, RAP1A, CDKN1A, MSH2, TERF2IP, APC, EGF, CCND1, ERBB2, PROC, CDK4, PIK3CB, PIK3CD, RASSF1, MGMT, TCEAL1, D4S234E, PKA | Background: B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma. Species: | Reactivity: | Cross reacts in Human and Rat. Does not react in Mouse. | Applications: | Uses: | Flow cytometry / FACS analysis, Immunohistochemistry, Immunoprecipitation and Western Blot | | Dilutions: | flow cytometry / FACS analysis 1:50, immunohistochemistry 1:250-500, immunoprecipitation 1:50, Western Blot 1:1000-5000 | | Positive Controls: | 1 positive controls | Packaging: | Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. | | Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. | | Preservative: | 0.01% Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | | | | |
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