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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-310233 |
NBP1-05219 |
68kDa Neurofilament Light Antibody//Rat/Chicken |
1/EA |
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문의 |
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Subject
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68kDa Neurofilament Light Antibody |
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Description
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/ Rat/Chicken |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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IF, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt | | Applications: | WB, IF | | Clonality: | Polyclonal | | | Gene: | NEFL | | Purity: | IgG purified | | Host: | Chicken | | | Specificity: | Neurofilament NF-L | | Publications: | Antibody has not yet been mentioned in a publication. | Details: Gene Symbol: NEFL | | 83613 (Rat) | | Related Genes: | NEFM, NF-H, NFASC, GFAP, MPZ, VIM, SOD1, KIF1B, LAMC2, MAPT, PRPH, NEFH, PMP22, MAP2, GJB1, MAD2L1BP, MBP, IGFALS, NF200, METAP2, HSPB1, ENO2, GAP-43, MFN2, PXMP2, NES, GCLC, GCA, GMCL1, GMCL1L, MTMR2, SYP, NGF, PHLDA2, BDNF, GARS, CDK5, DLG3, DES, MBL2 | Background: Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria. Species: Applications: | Uses: | Immunofluorescence and Western Blot | | Dilutions: | immunofluorescence 1:5000, Western Blot 1:10000 | Packaging: | Buffer: | PBS | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 10mM Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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