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| Cat. No |
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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-310239 |
NBP1-18582 |
6-Histidine Epitope Tag Antibody//WB/Rabbit |
1/EA |
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문의 |
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Subject
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6-Histidine Epitope Tag Antibody |
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Description
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/ WB/Rabbit |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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ELISA, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Applications: | WB | | Clonality: | Polyclonal | | | Purity: | Immunogen affinity purified | | Host: | Rabbit | | Details: Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Species: Applications: | Uses: | This is useful for the following applications:Western Blot: The antibody was tested using several His-tagged proteins expressed in insect cells and E. coli. The antibody detect only proteins with a C-terminal His-tag! The anti-His-tag is able to detect recombinant proteins in the conditioned media from insect cells and total lysate from E. coli. We recommend to use the IgG at a concentration of 0.5 ? 1 ug/ml in Western Blot analysis. | | Dilutions: | Western Blot 0.5-1 ug/ml, | Packaging: | Buffer: | Lyopholized | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | No Preservative | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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