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Description |
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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-310296 |
NBP1-05215 |
200kDa Neurofilament Heavy Antibody//Chicken, Rat/Rabbit |
1/EA |
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Subject
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200kDa Neurofilament Heavy Antibody |
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Description
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/ Chicken, Rat/Rabbit |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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ELISA, IF, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt, Ch | | Applications: | WB, IF, ELISA | | Clonality: | Polyclonal | | | Gene: | NEFH | | Purity: | Whole antisera | | Host: | Rabbit | | | Specificity: | Neurofilament NF-H (phosphate independent) | | Publications: | Antibody has not yet been mentioned in a publication. | Details: Gene Symbol: NEFH | | 24587 (Rat) | | Related Genes: | NEFM, NEFL, NFASC, SOD1, PRPH, GFAP, LDLR, FH, CFH, MAP2, MAPT, IGFALS, NES, REN, PRNP, AGT, MBP, UCHL1, METAP2, ACHE, HOXA9, MT1M, MAPK1, VIM, CLDN6, IL6, PSEN1, GPR158, PSEN2, RSPO4, PKP1, CDK5, ATP2A2, GAP-43, RANGAP1, MAPK9, CA3, SLC17A5, TAC1, LIF | Background: Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. The form of NF-H expressed in neurons varies in terms of the level of phosphorylation. Mammalian NF-H contains tandem repeats based on the sequence lysine serine proline (KSP), which may be present up to 60 times, with some variability between species. These KSP sequences are the sites of phosphorylation, and in axons most if not all of the serine residues are phosphorylated. In contrast, neurofilaments in dendrites and perikarya are not phosphorylated on these KSP sequences. Axonal neurofilaments are therefore quite different immunologically from those in perikarya and dendrites, and many antibodies stain only axonal or only dendritic/perikaryal neurofilaments. This antibody was raised against the KSP region of rat NF-H which was expressed in and purified from E. coli. It is similar to, but higher quality than, an antibody made and described many years ago (1). Since bacteria do not phosphorylate mammalian proteins, this protein resembles the NF-H form characteristic of perikaryal and dendritic neurofilaments. Accordingly this antibody stains dendritic and perikaryal neurofilaments particularly well. Species: | Reactivity: | Rat and Chicken | Applications: | Uses: | ELISA, Immunofluorescence and Western Blot | | Dilutions: | ELISA , immunofluorescence 1:1000, Western Blot 1:10000 | Packaging: | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | No Preservative | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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