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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-310348 |
NB100-66016 |
14-3-3 sigma Antibody//Bovine, Chicken/Rabbit |
1/EA |
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문의 |
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Subject
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14-3-3 sigma Antibody |
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Description
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/ Bovine, Chicken/Rabbit |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Sh, Rb, Rt, Mu, Hu, Ch, Bv | | Applications: | WB | | Clonality: | Polyclonal | | | Gene: | SFN | | Purity: | Whole antisera | | Host: | Rabbit | | | Specificity: | Recognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1. NB100-66016 may not react with recombinant proteins that are not N-acetylated. | | Publications: | Antibody has not yet been mentioned in a publication. | Details: | Isotype: | IgG | | Immunogen: | Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma.Peptide sequence: Ac.MERASLIQKAC | Gene Symbol: SFN | Entrez: | 2810 (Human) | | Related Genes: | TP53, CDKN1A, REXO2, CDKN2A, TCEAL1, D4S234E, MMP1, PAK3, YWHAQ, NFE2L2, AKT1, SLC12A9, MAPK1, TP63, CKAP4, TH, PTGS2, CCNB1, CDH1, BCL2, CASP3, TRIM25, GABPA, GSTP1, EGFR, SERPINB3, RARB, JUN, RASSF1, MMP3, CCND1, CDC2, MSLN, ERBB2, ESR1, BAX, PCNA, HDAC9, YWHAZ, MAPK8 | Background: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions. Species: | Reactivity: | Reacts with Human, Bovine, Chicken, Rat, Mouse, Rabbit and Sheep. | Applications: | Uses: | Western Blot In WB detects a band of approximately 30kDa in HEK293 cell lysates. | | Dilutions: | Western Blot | Packaging: | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 0.09% Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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