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Description |
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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-356912 |
SP6371P |
I Kappa B alpha (IKBa)//Human, Mouse, Rat/Rabbit |
1/EA |
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문의 |
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Subject
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I Kappa B alpha (IKBa) |
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Description
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/ Human, Mouse, Rat/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 50 ug | | Reactivity | Human, Mouse, Rat | | Presentation | Aff - Purified | | Applications | Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | NFKBIA, IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha | | Swiss Prot Num | P25963 | | Immunogen | Synthetic peptide corresponding to human/mice/rat I?ºB?± at the phosphorylation site of serines 32 and 36 | | Product Type | Antibodies | | Storage | Store the antibody at 2 - 8 °C or (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Karin, M et al (2000) Annu. Rev. Immunol. 18: 621-663. 2. Karin, M. et al. (2001) Nat. Immunol. 3: 221-227. | |
| Format | State: Liquid Ig fraction | | ? | Purification: Epitope affinity chromatography | | ? | Buffer System: 1x PBS (pH 7.4) containing 0.05 % sodium azide | |
| Specificity | This antibody is specific for I?ºB?± and is phosphorylated at the position of serines 32 and 36. The antibody was evaluated by ELISA, dot blot and Western blot. It only recognizes the phosphorylated serines 32 and 36 of I?ºB, not other phosphorylated sites or non-phosphorylated peptides. By immunoblot assay, this antibody recognizes ~40 kDa band in the TNF-treated Hela or 3T3 whole cell lysates. This immunoreactive band is abolished upon addition of the immunizing peptide. | | Application | ELISA (0.1 - 1.0 ?l/ml). Western blot (0.5 - 2.0 ?g/ml). Immunoprecipitation (3.0 - 5.0 ?g/extract from 10e7 cells). | | Background | NF-?ºB is a family of transcription factors, including p50, p52, p65, RelB, and C-Rel (1). It regulates the expression of a number of proteins involved in the regulation of cell survival and immune responses (2). The biological activity of NF-?ºB is tightly controlled by its inhibitor protein, I?ºB, which binds to NF-?ºB and sequesters it in the cytoplasm. There are three isoforms of the I?ºB: I?ºB?±, -?² and u“u. I?ºB?±, the well-characterized member of the I?ºB family contains both nuclear localization sequence and nuclear export signal that render to I?ºB?± the activity to rapidly shuttle between the nucleus and the cytoplasm. The I?ºB?± protein binds to NF-?ºB dimmers, masking their nuclear localization sequences and causing their cytoplasmic retention. After NF-?ºB positive stimuli, I?ºB kinase complex phosphorylates I?ºBs in two conserved serines (Ser-32 and Ser-36 in I?ºB?±), leading to ubiquitination and subsequent proteasomal degradation (1). | | Concentration | 0.2 mg/ml | |
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