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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-356913 |
SP6372P |
I Kappa B alpha (IKBa)//Human, Mouse, Rat/Rabbit |
1/EA |
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Subject
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I Kappa B alpha (IKBa) |
Description
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/ Human, Mouse, Rat/Rabbit |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 50 ug | Reactivity | Human, Mouse, Rat | Presentation | Aff - Purified | Applications | Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation | Host | Rabbit | PDF datasheet | view | Shipping to | Worldwide | Synonyms | NFKBIA, IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha | Swiss Prot Num | P25963 | Immunogen | Synthetic peptide corresponding to human I?¨¬B?¡¾ around serines 32 and 36 | Product Type | Antibodies | Storage | Store the antibody at 2 - 8 °C or (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | References | 1. Karin, M et al (2000) Annu. Rev. Immunol. 18: 621-663. 2. Karin, M. et al. (2001) Nat. Immunol. 3: 221-227. | |
Format | State: Liquid Ig fraction | ? | Purification: Epitope affinity chromatography | ? | Buffer System: 1x PBS (pH 7.4) containing 0.05 % sodium azide | |
Specificity | This antibody is specific for human I?¨¬B?¡¾. It was evaluated by ELISA, dot blot and Western blot. It only recognized the human I?¨¬B, not other phosphorylated or non-related peptides. By immunoblot assay, an immunoreactive band at ~40 kDa from Hela, 3T3, THP-1 whole cell lysate was detected. This immunoreactive band is abolished by the depletion of the immunizing peptide. | Application | ELISA: 0.1-1.0 ?g/ml. Western blot: 0.5-2.0 ?g/ml. Immunoprecipitaion (recommended): 3.0-5.0 ?g/extract from 10e7 cells. | Background | NF-?¨¬B is a family of transcription factors, including p50, p52, p65, RelB, and C-Rel (1). It regulates the expression of a number of proteins involved in the regulation of cell survival and immune responses (2). The biological activity of NF-?¨¬B is tightly controlled by its inhibitor protein I?¨¬B, which binds to and sequesters NF-?¨¬B in the cytoplasm. There are three isoforms of the I?¨¬B: I?¨¬B?¡¾, -?©÷ and -u. I?¨¬B?¡¾, the well-characterized member of the I?¨¬B family, contains both nuclear localization sequence and nuclear export signal that render to I?¨¬B?¡¾ the activity to rapidly shuttle between the nucleus and the cytoplasm. The I?¨¬B?¡¾ protein binds to NF-?¨¬B dimmers, masking their nuclear localization sequences and causing their cytoplasmic retention. After NF-?¨¬B positive stimuli, I?¨¬B kinase complex phosphorylates I?¨¬Bs in two conserved serines (Ser-32 and Ser-36 in I?¨¬B?¡¾), leading to ubiquitination and subsequent proteasomal degradation (1). | Concentration | 0.2 mg/ml | |
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