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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-352053 |
NBP1-20240 |
AKT1 Antibody//Human, Mouse,/Sheep |
1/EA |
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Subject
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AKT1 Antibody |
Description
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/ Human, Mouse,/Sheep |
Clonality
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Poly |
Company
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Novus biologicals |
Application
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IHC-P, Western Blot |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Hu, Rt, Mu | Applications: | WB, IHC | Clonality: | Polyclonal | | Gene: | AKT1 | Purity: | IgG purified | Host: | Sheep | | Specificity: | AKT1, AKT2, AKT3. | Publications: | Antibody has not yet been mentioned in a publication. | Details: Isotype: | IgG | Immunogen: | A synthetic peptide from the phospho S473 of mouse AKT (RAC-alpha serine/threonine-protein kinase, PKB, RAC) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, human and chicken AKT1. | Gene Symbol: AKT1 Entrez: | 207 (Human) | | 11651 (Mouse) | | 24185 (Rat) | Swiss Prot: | P31750, P47196, P31749 (Human) | Related Genes: | PIK3CG, PTK2B, MAPK1, PIK3CA, PIK3CB, PIK3CD, MAPK3, EPHB2, INS, MTOR, EGFR, PTEN, CASP3, VEGFA, INSR, MAPK8, EGF, MAPK12, NFKB1, MAPK14, GRAP2, AHSA1, AIMP2, SLC2A4, CDC42, SRC, BCL2L1, BCL2, NOS3, RHO, RHOD, CDKN1A, IRS1, TNF, ERBB2, PSMC4, JUN, PTK2, FOXO1, IRS-1 | Background: FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.; CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.; ENZYME REGULATION: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.; SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.; TISSUE SPECIFICITY: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis. Species: Reactivity: | Mouse, rat, human. | Applications: Uses: | Immunohistochemistry and Western Blot | Dilutions: | immunohistochemistry,Western Blot 10-50 ug/ml, | Notes: Reconstitute in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability. Packaging: Buffer: | Lyophilized in PBS | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | Preservative: | No Preservative | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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