Synonyms | E2F-1, RBBP3, RBBP-3, PBR3, RBAP-1, RBAP1, FocusOn059 |
Swiss Prot Num | Q01094 |
Immunogen | Synthetic peptide corresponding to amino acids 360-369 of human E2F1. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Pommier, Y. et al. (2006) Chk2 Molecular Interaction Map and Rationale for Chk2 Inhibitors. Clin. Cancer Res. 12: 2657 - 2661. |
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Format | State: Liquid purified IgG |
? | Buffer System: Phosphate buffered saline pH7.2 containing 0.09% Sodium Azide (NaN3) |
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Specificity | This antibody detects E2F1 phosphorylated at serine 364 (pSer364). |
Application | Immunohistochemistry on paraffin sections: 2 - 20?g/ml. ELISA: 1:10000 - 1:60000. Western Blot: 1:150 - 1:1000; detects a band of approximately 47kDa. |
Background | E2F1 is a transcription activator that binds DNA cooperatively with the differentiation regulated transcription factor proteins (DP). Together they bind to the promoter region of a number of genes whose products are involved in cell cycle regulation or DNA replication. E2F1 also binds to the retinoblastoma protein (RB1), which functions in the control of cell-cycle progression from G1 to S phase. E2F1 can also mediate p53-dependent and independent apoptosis, and phosphorylation of E2F1 at serine 364 results in its stabilisation and apoptosis induction. E2F1 is a target of the transforming proteins of small DNA tumour viruses and increased expression of this protein has been reported in a variety of cancers. |
Caution | (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. |
Concentration | 0.5mg/ml |
Focuson | 059 Antibodies to E2F Transcription Factor Family |