Synonyms | BING2, DAP6, hDaxx, ETS1-associated protein 1, EAP1 |
Swiss Prot Num | Q9UER7 |
Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S671 of human DAXX. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Cantrell, S.R., et al., J. Virol. 79(12):7792-7802 (2005). 2. Greger, J.G., et al., J. Virol. 79(8):4610-4618 (2005). 3. Chang, C.C., et al., J. Biol. Chem. 280(11):10164-10173 (2005). 4. Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004). 5. Gostissa, M., et al., J. Biol. Chem. 279(46):48013-48023 (2004). |
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Format | State: Liquid purified Ig fraction. |
? | Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification. |
? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. |
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Specificity | This antibody detects DAXX pSer671. |
Add. information | Molecular weight: 81373 Da |
Application | ELISA: 1/1,000. Dot Blot: 1/500. |
Background | DAXX is proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. DAXX seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. This protein down-regulates basal and activated transcription, and it also seems to act as a transcriptional co-repressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. DAXX modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. |
Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Concentration | 0.25 mg/ml |