Synonyms | GRB2-associated-binding protein 1; GRB2-associated binder 1; GAB1; |
Swiss Prot Num | Q13480 |
Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y627 of human GAB1. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Podar, K., et al., J. Biol. Chem. 279(20):21658-21665 (2004). 2. Ren, Y., et al., J. Biol. Chem. 279(9):8497-8505 (2004). 3. Kapoor, G.S., et al., Mol. Cell. Biol. 24(2):823-836 (2004). 4. Nakaoka, Y., et al., Circ. Res. 93(3):221-229 (2003). 5. Holgado-Madruga, M., et al., Mol. Cell. Biol. 23(13):4471-4484 (2003). |
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Format | State: Liquid purified Ig fraction. |
? | Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. |
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Specificity | This antibody detects Gab1 pTyr627. |
Add. information | Molecular weight: 76616 Da |
Application | ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100. |
Background | The Grb2-associated binder 1 (Gab1) is a multisubstrate scaffold/adapter protein that contains a pleckstrin homology domain at the NH2 terminus and multiple tyrosyl residues and proline-rich motifs for association with src homology 2 (SH2)-containing and SH3-containing proteins. Gab1, as well as its close relatives Gab2 and Gab3, serves to couple signaling between cell surface receptors for growth factors and cytokines with a variety of downstream targets, including Shp2 tyrosine phosphatase, phosphatidylinositol 3-kinase (PI3K), and phospholipase C-gamma. Ablation of the Gab1 gene in mice resulted in embryonic death in homozygous mutants, with developmental defects in the placenta, heart, and skin. Biochemical analysis of Gab1-/- fibroblast cells suggested a positive role of Gab1 in coupling the signal relay between cytokine receptors and the ERK pathway. |
Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Concentration | 0.25 mg/ml |