Synonyms | Review 022 |
Swiss Prot Num | P19969 |
Immunogen | Synthetic peptide corresponding to the C-Terminal region of the alpha 5 subunit of rat GABAA receptor |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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Format | State: Liquid purified Ig |
? | Purification: Affinity chromatography |
? | Buffer System: 10mM HEPES, pH7.5 containing 0.09% Sodium Azide, 0.01% Bovine Serum Albumin and 50% Glycerol |
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Specificity | This antibody is specific for the alpha 5 subunit of gamma-aminobutyric acid A (GABA A) receptor. |
Application | Western Blot: 1:1000; detects a band of approximately 55kDa in rat brain membrane fractions. |
Background | GABA A receptors are GABA gated chloride channels, which are important mediators of neuronal inhibition in the mammalian central nervous system. Activation of the GABA A site results in increased intracellular chloride concentration and hyperpolarization of the cell. GABA A receptors are pentameric structures assembled from a family of distinct subunits. At least fourteen distinct subunits have been identified, which show distinct patterns of temporal and spatial expression. GABA A receptors may be modulated by several drug types including benzodiazepines and barbiturates, and by phosphorylation by protein kinase C (PKC) and protein kinase A (PKA). |
Caution | (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. |
Review | r022 GABA A Receptor Regulator of Excitability and Drug Ta |