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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-352477 |
NB110-93474 |
alpha Tubulin 4a Antibody (DM1A)//Am, Bovine,/Mouse |
1/EA |
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Subject
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alpha Tubulin 4a Antibody (DM1A) |
Description
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/ Am, Bovine,/Mouse |
Clonality
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Mono |
Company
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Novus biologicals |
Application
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ICC, IHC-P, Western Blot |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Fu, Am, Ye, Rt, Mu, Hu, Ch, Bv | Applications: | ICC, WB, IHC | Clonality: | Monoclonal | | Gene: | TUBA4A | Purity: | IgG purified | Host: | Mouse | | Specificity: | The is specific for alpha-tubulin in immunoblotting assays and may be used for localization of alpha-tubulin in cultured cells or tissue sections. The reacts best with chicken fibroblasts. | Publications: | Antibody has been mentioned in at least 1 publication. | Details: Clone: | DM1A | Isotype: | IgG1 | Immunogen: | microtubules from chicken embryo brain. | Gene Symbol: TUBA4A Entrez: | 7277 (Human) | | 22143 (Mouse) | | 500929 (Rat) | Related Genes: | GAPDH, B2M, BDNF, TBP, RUNX1, VCL, PGK1, RABGAP1L, SERPINB4, SERPINB3, PPIA, PRDX1, S100A9, SOD2, HPRT1, EZR, TUBA1A, EPB41L3, TPM1, H2, PF4V1, E11, MASCOT, SLC24A3, STXBP1, EEF1A1, PSMD1, MYC, PRM1, TUFM, SGCA, RHCG, ARNTL, SEPT3, DNASE1, ITGB3, TUBA3C, CSTB, PHB, RPLP1 | Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Species: Reactivity: | This antibody is reactive against Chicken, Human, Bovine, Rat, Mouse, Amphibian, Yeast, Fungi. | Applications: Uses: | This antibody is useful for: Western Blot 0.5-1 ìg/mL, using total tissue extract from chicken gizzard. This antibody detects a band at ~50 kDa immunocytochemistry: 0.5-1 ìg/mL using cultured chicken fibroblasts (CFB) immunohistochemistry immunoprecipitation microarray | Dilutions: | immunohistochemistry , Western Blot 0.5-1 ug/ml, Immunocytochemistry 0.5-1 ug/ml, | Concentrations: | 1.0 mg/ml | Positive Controls: | 1 positive controls | Packaging: Buffer: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA. | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | Preservative: | 15mM Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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