| Catalog Number | GTX11512 |
| Product Name | E Cadherin [DECMA-1] antibody |
| Product Description | Rat monoclonal [DECMA - 1] to E Cadherin |
| Synonyms | 12550 | Cdh1 | AA960649 | E-cadherin | Ecad | MGC107495 | UVO | Um | uvomorulin | 999 | CDH1 | 137215 | P12830 | Arc-1 | CDHE | ECAD | LCAM | UVO | |
| Background | Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+. |
| Clonality | Monoclonal |
| Clone No | DECMA-1 |
| Host | Rat |
| Isotype | IgG1 |
| Immunogen | Mouse embryonal carcinoma cell line PCC4 Aza RI. |
| Antigen Species | Mouse |
| Tested Applications | Immunofluorescence, IHC (Frozen sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | IF: Use at a dilution of 1/1600. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 106 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Mouse and Dog. Not yet tested in other species. |
| Positive Controls | Tested with MDCK cells |
| Target | E Cadherin |
| Purification | Ascites |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Raw Ascites |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
