| Catalog Number | GTX15148 |
| Product Name | E Cadherin antibody |
| Product Description | Rabbit polyclonal to E Cadherin |
| Synonyms | 999 | CDH1 | 137215 | P12830 | Arc-1 | CDHE | ECAD | LCAM | UVO | |
| Background | One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Recombinant fragment: IFFCERNPKP QVINIIDADL PPNTSPFTAE LTHGASANWT IQYNDPTQES IILKPKMALE VGDYKINLKL MDNQNKDQVT TLEVSVCDCE GAAGVCRKAQ PVEAGLQI , corresponding to amino acids 600-707 of Human E Cadherin. |
| Antigen Species | Human |
| Tested Applications | IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IHC-P: Use at a dilution of 1/30 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Human. Does not cross-react with Chicken. Not yet tested in other species. |
| Positive Controls | Skin |
| Target | E Cadherin |
| Form Supplied | Liquid |
| Concentration | 0.2 mg/ml |
| Purification | Immunogen affinity purified |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
Image:GTX15148_IHC1.jpgimg25308.jpgImmunohistochemical staining of formalin fixed paraffin embedded human skin using GTX15148. Print:Print
