| Catalog Number | GTX20167 |
| Product Name | E Cadherin [6F9] antibody |
| Product Description | Mouse monoclonal [6F9] to E Cadherin |
| Synonyms | 999 | CDH1 | 137215 | P12830 | Arc-1 | CDHE | ECAD | LCAM | UVO | |
| Background | One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcinomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-Cadherin expression. Therefore, the expression of E-Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. |
| Clonality | Monoclonal |
| Clone No | 6F9 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Affinity purified 80kD extracelluilar fragments of E-Cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells. |
| Antigen Species | Human |
| Tested Applications | Immunocytochemistry, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | WB: Use at a dilution of 1:50-1:500. ICC: Use at an assay dependent concentration. IHC-Fr: Use at an assay dependent concentration. IHC-P: Use at an assay dependent dilution. Permeabilization in 0.2% Triton X-100 and pronase digestion is required. Recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. In frozen and paraffin sections, for dilution use a PBS buffer containing 0.1mM CaCl2 and 0.1mM MgCl2. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Recognises human E-cadherin, an epithelial cell adhesion molecule located on the lateral border of epithelial cells. In blots, the antibody reacts with the 80 kD and 120 kD bands of E-cadherin.In IHC the reaction is confined to the lateral borders of the epithelial cells, or more intensely in the junctional complex in the small intestine. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Target | E Cadherin |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified. |
| Storage Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
