| Catalog Number | GTX20777 |
| Product Name | E Cadherin [67A4] antibody |
| Product Description | Mouse monoclonal [67A4] to E Cadherin |
| Synonyms | 999 | CDH1 | 137215 | P12830 | Arc-1 | CDHE | ECAD | LCAM | UVO | |
| Background | One of the epithelial cell adhesion molecules, E Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E Cadherin expression. Therefore, the expression of E Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm. |
| Clonality | Monoclonal |
| Clone No | 67A4 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Human breast carcinoma cell line T471. |
| Antigen Species | Human |
| Tested Applications | IHC (Frozen sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Suitable for frozen tissue sections only |
| Specificity | This antibody is specific for the 123 kDa E-cadherin molecule, expressed on non-neuronal epithelial cells. This antibody reacts with tumor cell lines such as breast carcinoma cells. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Target | E Cadherin |
| Form Supplied | Liquid |
| Purification | Tissue culture supernatant |
| Storage Buffer | Tissue culture supernatant with sodium azide |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
