| Catalog Number | GTX21416 |
| Product Name | E Cadherin [HECD-1] antibody |
| Product Description | Mouse monoclonal [HECD-1] to E Cadherin |
| Synonyms | 999 | CDH1 | 137215 | P12830 | Arc-1 | CDHE | ECAD | LCAM | UVO | |
| Background | E-cadherin is a transmembrane glycoprotein that mediates epithelial cell-to-cell adhesion. The loss of E-cadherin can result in the disruption of cell clusters. It is therefore, postulated that E-cadherin may function as a tumor suppressor protein. The loss of E-cadherin has been associated with metastasis and poor prognosis in invasive breast cancer and can help differentiate between ductal and lobular neoplasms of the breast. It has also been shown to be an independent predictor in the disease progression in bladder cancer. |
| Clonality | Monoclonal |
| Clone No | HECD-1 |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Breast tumor cell line MCF-7 |
| Antigen Species | N/A |
| Tested Applications | Immunofluorescence, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections). The usefulness of this product in other applications has not been determined. |
| Application Note | IF: Use at a dilution of 1/50. IHC-P: Use at a dilution of 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, dilute 1:50-100 and incubate for 30 minutes at RT. IHC-Fr: Use at a dilution of 1/50 - 1/100. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Positive Controls | Breast Cancer |
| Target | E Cadherin |
| Form Supplied | This antibody is produced in tissue culture supernatant, concentrated and then placed in to PBS pH7.3 with a protein carrier and a proprietary preservative that is not sodium azide or thimerosal. Approximately 10 mg/ml total Ig concentration. |
| Purification | n/a |
| Storage Buffer | Preservative: Proprietary, not sodium azide or thimerosal. Constituents: PBS, Tissue Culture Supernatant. pH 7.3; Protein carrier. |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
