| Catalog Number | GTX28997 |
| Product Name | E Cadherin [Sec21] antibody |
| Product Description | Mouse monoclonal [Sec21] to E Cadherin |
| Synonyms | 999 | CDH1 | 137215 | P12830 | Arc-1 | CDHE | ECAD | LCAM | UVO | |
| Background | Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighboring cells. In addition, E-cadherin can interact heterophilically with integrin aEb7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently down-regulated, which is consistent with its function of an invasion suppressor in normal epithelia. |
| Clonality | Monoclonal |
| Clone No | Sec21 |
| Host | Mouse |
| Isotype | IgG |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 30-43 of Human E Cadherin. |
| Antigen Species | Human |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | WB: 1/100 - 1/1000. |
| Specificity | This antibody recognizes both the 120 kD E-cadherin and its 80 kDa trypsin-resistant extracellular part. The epitope is present within amino acid residues 30-43 of mature human E-cadherin. |
| Species Cross Reactivity | Human - Not yet tested in other species |
| Target | E Cadherin |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified. |
| Storage Buffer | Phosphate-buffered saline, containing 0.1% sodium azide |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
