Immunogen | F(ab')2 fragment of rat IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Product Type | Antibodies |
Storage | Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
References | 1. Hunt, S.V. and Fowler, M.H. (1981) A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras. Cell Tissue Kinet 14: 445-464. 2. Bukovsky, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy. Immunology. 52:631-640. 3. Balogh, P. et al. (2002) Simultaneous blockade of Fcgamma receptors and indirect labeling of mouse lymphocytes by the selective detection of allotype-restricted epitopes on the kappa chain of rat monoclonal antibodies. Cytometry. 47:107-110. |
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Format | Reconstitution: Restore with 1.0 ml distilled water |
? | State: Lyophilized purified IgG |
? | Label: R. Phycoerythrin (RPE) |
? | Purification: Affinity chromatography on Protein G |
? | Buffer System: PBS, pH7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin |
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Specificity | This antibody recognises kappa chains, showing a marked preference for binding to the Igka allotype which is present in AGUS, AO, AUG, BN, LEW, LOU, PVG and WAG strains but not in ACI, DA, F344, MSU or SHR rat strains. |
Application | Flow cytometry. |