| Synonyms | T4/Leu-3 |
| Swiss Prot Num | Q50EU7 |
| Immunogen | 293T cells expressing Pekin duck CD4. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line. |
| Product Type | Antibodies |
| Recommended Isotype Control | AM03096PU-N |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev. and Comp. Immunol. 29: 733-748. 2. Higgins, D.A. and Teoh, C.S. (1988) Duck lymphocytes. Culture conditions for optimum transformation response to phytohaemagglutinin. J. Immunol. Methods. 21: 135-145. |
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| Format | State: Liquid purified IgG fraction. |
| ? | Purification: Affinity Chromatography on Protein G. |
| ? | Buffer System: PBS containing 0.09% Sodium Azide as preservative. |
|
| Specificity | This antibody recognises CD4. Clone Du CD4-2 can be used to identify duck T helper cells. |
| Application | Flow Cytometry. Immunoprecipitation. |
| Background | CD4 is shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells. (1) |
| Concentration | 1.0 mg/ml |
