| Synonyms | S100 Calcium binding protein, S-100 protein, Astrocyte Marker, FocusOn041 |
| Immunogen | Peptide derived from C-terminal sequence of human S100-A1 protein. |
| Product Type | Antibodies |
| Recommended Isotype Control | PP501P |
| Storage | Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Protocols | IHC-P -PROTOCOL 1. Deparaffinize the section in 3 changes of xylene, 5 minutes each. 2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each 3. Rinse in distilled water 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes 5. Wash in distilled water for 5 minutes 6. Wash in 0.05 M Tris-HCl , pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes 7. Incubate the section with primary antibody diluted in Buffer A at the dilution 1/100-1/200 for 1 hour in the closed wet chamber 8. Wash twice 5 minutes with buffer A 9. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB) 10. Wash twice 5 minutes with buffer A 11. Apply the chromogen (DAB), 10 minutes 12. Wash in water for 10 minutes 13. Stain in hematoxylin for 5 minutes 14. Wash in water for 10 minutes 15. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each 16. Wash the section in 2 changes of xylene for 2 minutes each. 17. Mount the slide for observation. |
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| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Immunoaffinity Chromatography. |
| ? | Buffer System: 20 mM Tris-HCl, pH 8.0 containing 20 mg/ml BSA as stabilizer and 0.05% Sodium Azide as preservative. |
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| Specificity | This antibody recognises S100. |
| Application | Immunohistochemistry on Paraffin Sections (1/100-200). |
| Background | S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumours of the skin, and in Langerhans cells in the skin. |
| Focuson | 041 Antibodies to S100 Family |
