Swiss Prot Num | Q9H9S0 |
Immunogen | Highly pure (> 98 %) recombinant human Nanog |
Product Type | Antibodies |
Storage | Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
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Format | Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml. |
? | State: Sterile filtered lyophilized Ig fraction |
? | Label: Biotin |
? | Purification: Affinity chromatography |
? | Buffer System: PBS, pH 7.2 |
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Specificity | This antibody detects Nanog. |
Application | ELISA: Direct: To detect hNanog (using 100 ?¨ùl/well antibody solution) a concentration of 0.25 - 1.0 ?¨ùg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Sandwich: To detect hNanog (using 100 ?¨ùl/well antibody solution) a concentration of 0.25 - 1.0 ?¨ùg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human Nanog as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Western blot: To detect hNanog this antibody can be used at a concentration of 0.1 - 0.2 ?g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |