Synonyms | NAPSA, NAP1, Napsin-1,Aspartyl protease 4, TA01/TA02 |
Swiss Prot Num | O96009 |
Immunogen | Synthetic peptide from N terminus of Human Napsin A |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch. |
References | 1. Chuman et. FEBS Lett 462: 129, 1999. 2. Dejmek et al. Diag Cytoplathol 35: 493, 2007. 3. Ueno et al. Br. J. Cancer 88, 1229, 2003. |
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Format | State: Liquid purified Ig fraction containing Sodium azide as preservative. |
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Specificity | Napsin A and TTF-1 reactivities were determined immunohistochemically on formalin-fixed paraffin embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). TTF-1 and Napsin A were positive in 8/12 and 10/12 pulmonary adenocarcinomas, respectively. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive. This antibody may be a useful tool as a tumor marker for primary lung adenocarcinoma. |
Add. information | Cellular Localization: Cytoplasmic |
Application | Immunohistochemistry on Paraffin Sections: Use at 1/50-1/100 dilution in an ABC method (30 minutes at room temperature). However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Lung Adenocarcinoma. |
Background | Napsin is found in two isoforms, A and B, with highly homologous gene sequences. Napsin A has a molecular weight of 35.0 kDa and is also known as TA02. Napsin A is an aspartic proteinase which is expressed in the lung and involved in processing surfactant protein B (SP-B). It is also expressed in the kidney. |