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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-372043 |
AP01571PU-N |
DARPP-32//Human, Mouse, Rat/Rabbit |
1/EA |
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¹®ÀÇ |
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Subject
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DARPP-32 |
Description
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/ Human, Mouse, Rat/Rabbit |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 100 ug | Reactivity | Human, Mouse, Rat | Presentation | Aff - Purified | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | Host | Rabbit | PDF datasheet | view | Shipping to | Worldwide | Synonyms | Protein Phosphatase 1 regulatory subunit 1B, PPP1R1B, DARPP32, DARPP-32 | Swiss Prot Num | Q9UD71 | Product Type | Antibodies | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | |
Format | State: Liquid purified Ig | ? | Purification: Affinity chromatography | ? | Buffer System: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. | |
Specificity | p-DARPP-32 (pThr75) antibody detects endogenous levels of p-DARPP-32 protein. | Application | ELISA: 1:40000~1:60000. Western Blot: 1:500~1:1000. Immunohistochemistry: 1:50~1:200. | Background | Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinsonu¢âs disease and drug abuse. DARPP-32 (for dopamine and adenosine 3u¢â,5u¢â-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on Thr 34 occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2- like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32 at Thr 34. Phosphorylated DARPP-32 at Thr 34 is a potent inhibitor of PP-1. Phosphorylation of DARPP-32 on Ser 137 by casein kinase inhibits the dephosphorylation of Thr 34 by calcineurin. Phosphorylation of DARPP-32 on Thr 75 by CdK5 inhibits PKA by a competitive mechanism in vitro. Decreasing the phosphorylaton of DARPP-32 Thr 75 increases the dopamine-induced phosphorylation of PKA substrates. | Concentration | 1,0 mg/ml | |
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