| Synonyms | E2F-1, RBBP3, RBBP-3, PBR3, RBAP-1, RBAP1, FocusOn059 |
| Swiss Prot Num | Q01094 |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S332 of human E2F1. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005). 2. Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). 3. Joshi, B., et al., Oncogene 24(13):2204-2217 (2005). 4. Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004). 5. Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004). |
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| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification. |
| ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. |
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| Specificity | This antibody detects E2F1 pSer332. |
| Add. information | Molecular weight: 46920 Da |
| Application | ELISA: 1/1,000. Dot Blot: 1/500. |
| Background | E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. |
| Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Concentration | 0.25 mg/ml |
| Focuson | 059 Antibodies to E2F Transcription Factor Family |
