| Synonyms | CD8 alpha, MAL, CD8 antigen 32 kDa chain, OX-8 Membrane Antigen |
| Swiss Prot Num | P01732 |
| Immunogen | 293T cells expressing Pekin Duck CD8 alpha. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line. |
| Product Type | Antibodies |
| Recommended Isotype Control | SM12P, AM03110PU-N |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev. and Comp. Immunol. 29: 733-748. 2. Higgins, D.A. and Teoh, C.S. (1988) Duck lymphocytes. Culture conditions for optimum transformation response to phytohaemagglutinin. J. Immunol. Methods. 21: 135-145. |
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| Format | State: Liquid purified IgG |
| ? | Purification: Affinity Chromatography on Protein G. |
| ? | Buffer System: PBS containing 0.09% Sodium Azide as preservative. |
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| Specificity | This antibody recognises CD8 alpha. Double-staining with clone Du CD8-1 and duck IgY light chain clone 14A3 (product code SM2274P), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+/14A3+ cells in duck bursa. (1) |
| Application | Flow Cytometry. Immunoprecipitation. |
| Background | CD8 alpha shown to be expressed by thymocytes, splenocytes, peripheral lymphoid cells and the vast majority of bursal cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs: the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA). |
| Concentration | 1.0 mg/ml |
