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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-368451 |
AP01721PU-N |
Acetyl-CoA carboxylase 1//Human, Mouse, Rat/Rabbit |
1/EA |
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Subject
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Acetyl-CoA carboxylase 1 |
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Description
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/ Human, Mouse, Rat/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 100 ug | | Reactivity | Human, Mouse, Rat | | Presentation | Aff - Purified | | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | ACACA, ACAC, ACC1, ACCA | | Swiss Prot Num | Q13085 | | Product Type | Antibodies | | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch. | |
| Format | State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). | | ? | Purification: Affinity Chromatography using epitope-specific immunogen. | | ? | Buffer System: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative. | |
| Specificity | This antibody detects endogenous levels of Acetyl-CoA Carboxylase pSer80 protein. | | Application | ELISA: 1/1000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. | | Background | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACC?± (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACC?² (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACC?± is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACC?±, which maps to human chromosome 17, encodes the 265 kDa ?± form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACC?² is homologous to that of ACC?± except for an additional peptide of about 150 amino acids at the N-terminus. Western blot (WB) analysis of p-Acetyl-CoA Carboxylase (S80) antibody in extracts from 293 cells treated with EGF 200ng/ml 5' or NIH/3T3 cells treated with PMA 250ng/ml 5' | | Concentration | 1.0 mg/ml | |
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