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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-368483 |
AP12263PU-N |
ACSL4//Human/Rabbit |
1/EA |
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문의 |
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Subject
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ACSL4 |
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Description
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/ Human/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.1 mg | | Reactivity | Human | | Presentation | Purified | | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | | Isotype | Ig | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | ACS4, FACL4, LACS4 | | Swiss Prot Num | O60488 | | Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human FACL4. | | Product Type | Antibodies | | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | |
| Format | State: Liquid purified Ig fraction. | | ? | Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | | ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. | |
| Specificity | This antibody is specific to ACSL4/FACL4 (Center). | | Add. information | Predicted Molecular weight: 79188 Da | | Application | ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. | | Background | Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligase (FACL), converts free long chain fatty acids into fatty acyl-CoA esters, key intermediates in the synthesis of complex lipids. The FACL4 gene encodes a form of LACS and is expressed in several tissues, including brain. FACL4 cDNA from brain encodes a gene product that shows preference for arachidonic acid as a substrate when expressed in mammalian cells.1 The sequence of the predicted 670-amino acid human protein is 97% identical to that of rat ACS4. FACL4 is highly expressed in adult human brain, especially in the cerebellum and hippocampus, similar to the mouse.2 A strong cytoplasmic staining was found in the Purkinje and granular cells of the cerebellum and the pyramidal layer of hippocampus, indicating that FACL4 is specifically expressed in neurons and not in glial cells. Two patients with Alport syndrome, elliptocytosis, and mental retardation carried a large deletion of the COL4A5 region that included FACL4.3 The absence of FACL4 might play a role in the development of mental retardation or other signs associated with Alport syndrome. Two point mutations, 1 missense and 1 splice site change, were reported in the FACL4 gene in 2 families with nonspecific mental retardation.2 Analysis of enzymatic activity in lymphoblastoid cell lines of affected individuals revealed low levels compared with normal cells, indicating that both mutations are null mutations. | | Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. | | Concentration | 0.25 mg/ml | |
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