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| Cat. No |
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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-373091 |
AP12545PU-N |
H3//Human/Rabbit |
1/EA |
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문의 |
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Subject
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H3 |
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Description
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/ Human/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.1 mg | | Reactivity | Human | | Presentation | Purified | | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | | Isotype | Ig | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | Histone H3.1; H3/a; H3/b; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; | | Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human H3. | | Product Type | Antibodies | | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003). 2. Deng, L., et al., Virology 289(2):312-326 (2001). 3. Deng, L., et al., Virology 277(2):278-295 (2000). 4. El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998). 5. Albig, W., et al., Hum. Genet. 101(3):284-294 (1997). | |
| Format | State: Liquid purified Ig fraction. | | ? | Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | | ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. | |
| Specificity | This antibody detects H3 pSer10. | | Add. information | Molecular weight: 15273 Da | | Application | ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. | | Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The gene for this protein is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. The gene is found in the large histone gene cluster on chromosome 6p22-p21.3. | | Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. | | Concentration | 0.25 mg/ml | |
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