| Synonyms | C-C motif chemokine 22, ABCD-1, Scya22, Small-inducible cytokine A22 |
| Swiss Prot Num | O88430 |
| Immunogen | Highly pure (> 98 %) recombinant murine MDC |
| Product Type | Antibodies |
| Storage | Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
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| Format | Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml. |
| ? | State: Sterile filtered lyophilized Ig fraction |
| ? | Purification: Immunoaffinity chromatography |
| ? | Buffer System: PBS, pH 7.2 |
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| Specificity | This antibody detects MDC. |
| Application | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mMDC (100 ng/ml), a concentration of 6 - 9 ?g/ml is required. ELISA: Indirect: To detect mMDC by indirect ELISA (using 100 ?¼l/well antibody solution) a concentration of 0.5 - 2.0 ?¼g/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC. Sandwich: To detect mMDC by sandwich ELISA (using 100 ?¼l/well antibody solution) a concentration of 0.5 - 2.0 ?¼g/ml is required. This In conjunction with Biotinylated Anti-Murine MDC as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMDC. Western Blot: To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ?g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
