Catalog Number | GTX22913 |
Product Name | alpha COP I antibody |
Product Description | Rabbit polyclonal to alpha COP I |
Synonyms | 304978 | Copa | |
Background | Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Synthetic peptide: MLTKFETKSARVKGLSFHP-C, corresponding to amino acids 1-19 of Rat alpha COP I. |
Antigen Species | Rat |
Tested Applications | Immunocytochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | Immunocytochemistry: use at 3 ¥ìg/ml. Western Blot: use at 1 ¥ìg/ml. By Western blot, this antibody detects an ~140 kDa protein representing COPI alpha from PC-12 cell extract. This antibody also detects an unidentified protein at ~100 kDa. Immunocytochemical staining of alpha COP I using GTX22913 results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. |
Specificity | Detects coatomer-protein I alpha (COP I alpha). |
Species Cross Reactivity | Cross-reacts with Human and Rat. Not yet tested in other species. |
Target | alpha COP I |
Form Supplied | Liquid |
Purification | Immunogen affinity purified |
Storage Buffer | PBS, 1 mg/ml BSA, 0.05% sodium azide |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
Image:GTX22913.jpgimg3715.jpgImmunolocalization of COPI alpha in HeLa cells using GTX22913. Print:Print