| Catalog Number | GTX25776 |
| Product Name | Dab1 phospho S491 antibody |
| Product Description | Rabbit polyclonal to Dab1 ( phospho S491 ) |
| Synonyms | 115209 | OMA1 | Q96E52 | 2010001O09Rik | DAB1 | FLJ33782 | MPRP-1 | YKR087C | ZMPOMA1 | 1600 | DAB1 | 603448 | O75553 | |
| Background | Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic phosphopeptide derived from the region of human Dab1 that contains serine 491. |
| Antigen Species | Human |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | WB: Use at a dilution of 1/1000. Detects a band of approximately 80 kDa. When examining phosphorylation of endogenous Dab1 protein we recommend that Dab1 protein first be immunoprecipitated. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Species Cross Reactivity | Cross-reacts with Human and Mouse. Expected to cross-react with Chicken (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Positive Controls | Human Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein |
| Target | Dab1 |
| Purification | Protein A purified |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
