| Catalog Number | GTX13626 |
| Product Name | E. coli antibody |
| Product Description | Rabbit polyclonal to E.coli |
| Synonyms | Escherichia coli |
| Background | Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Mixtures of all antigenic serotypes. |
| Tested Applications | ELISA, Immunohistochemistry. The usefulness of this product in other applications has not been determined. |
| Application Note | ELISA: 1/2000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12. IHC: Use at an assay dependent dilution. Immunblotting: Use at an assay dependent dilution. Fluorescence miscroscopy: Use at an assay dependent dilution. GTX13626 may be used in place of neat antiserum in almost any appropriate antibody-based technique. Will remove E. coli proteins from recombinant preparations. GTX13626 is suitable for conjugation purposes. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | GTX13626 reacts with all ""O"" and ""K"" antigenic serotypes of Escherichia coli. |
| Species Cross Reactivity | Cross-reacts with all "O" and "K" antigenic serotypes of Escherichia coli.Antiserum is not absorbed for other members of the Enterobacteriaciae. Not yet tested in other species. |
| Target | E. coli |
| Cellular Localization | Whole cell |
| Form Supplied | Liquid |
| Purification | IgG fraction |
| Purification Note | This product consists of the purified IgG fraction of the above antiserum prepared by methods developed to a purity of >95%. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 0.01M PBS. pH 7.2 |
| Storage Instruction | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
