Catalog Number | GTX11837 |
Product Name | E2F1 antibody |
Product Description | Rabbit polyclonal to E2F1 |
Synonyms | 1869 | E2F1 | 189971 | Q01094 | E2F-1 | RBBP3 | RBP3 | |
Background | E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Synthetic peptide - conjugated to KLH by a Glutaraldehyde linker, corresponding to the C-terminal amino acids 310-329 of Human E2F 1. |
Antigen Species | Human |
Tested Applications | Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | IP: Use at a concentration of 10 ¥ìg/ml. This concentration will immunoprecipitate E2F1 from extracts of transfected cells expressing recombinant human E2F1. WB: Use at a concentration of 2.5 ¥ìg/ml. This concentration is determined using a whole extract of transfected cells expressing recombinant human E2F1, or in vitro translated E2F1 protein. Detects a band of approximately 50 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
Species Cross Reactivity | Human - Not yet tested in other species |
Target | E2F1 |
Cellular Localization | Nuclear |
Form Supplied | Liquid |
Purification | Immunogen affinity purified |
Purification Note | From polyclonal serum |
Storage Buffer | 10mM PBS. pH 7.4, containing 1% BSA and 15mM sodium azide |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
Notes | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours." |