Catalog Number | GTX14769 |
Product Name | E2F1 antibody |
Product Description | Rabbit polyclonal to E2F1 |
Synonyms | 1869 | E2F1 | 189971 | Q01094 | E2F-1 | RBBP3 | RBP3 | |
Background | E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F1 overexpression can also drive cells into apoptosis. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Synthetic peptide: RFLELLS, corresponding to amino acids 138-144 of Human E2F1. |
Antigen Species | Human |
Tested Applications | Immunohistochemistry, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 53 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | The antibody does not cross reacts with other proteins of the E2F family members. |
Species Cross Reactivity | Human - Not yet tested in other species |
Target | E2F1 |
Cellular Localization | Nuclear |
Purification | Immunogen affinity purified |
Storage Instruction | Keep as concentrated solution. Store at 4¨¬C short term. For extended storage aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |