Catalog Number | GTX77774 |
Product Name | E2F1 antibody |
Product Description | Rabbit Polyclonal Antibody to E2F1 |
Synonyms | 1869 | E2F1 | 189971 | Q01094 | E2F-1 | RBBP3 | RBP3 | |
Background | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. |
Clonality | Polyclonal |
Host | Rabbit |
Isotype | IgG |
Immunogen | Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of human E2F1 |
Antigen Species | Human |
Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | WB: Suggested dilution at 1 ¥ìg/ml in 5% skim milk / PBS buffer. Optimal dilutions should be determined by end-user. |
Species Cross Reactivity | Human - Not yet tested in other species |
Target | E2F1 |
Form Supplied | Liquid |
Purification | Protein A/G affinity purified |
Purification Note | From polyclonal serum |
Storage Buffer | PBS with 2% sucrose |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |
Notes | For research use only not for diagnostic, human, or veterinary use." |
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